Use of two biomarkers of renal ischemia to assess machine-perfused non-heart-beating donor kidneys.

aspect to take into consideration when choosing among reagents. Reconstituting a fresh vial of reagent daily dramatically increases costs when the majority of a previous vial is discarded. All reagents tested were relatively inexpensive, costing an average of US $3.00/mL (list price). In summary, all assays detected hypofibrinogenemia, although the BioMerieux reagent yielded slightly prolonged values for the normal sample on one day. All reagents were sensitive to heparin, although the BioData reagent was too sensitive, with a terminal clotting time at heparin concentrations 100 IU/L. Use of this reagent would make it difficult to monitor a patient receiving therapeutic amounts of heparin. In contrast, the BioMerieux, BioPool, and Sigma Diagnostics reagents produced measurable clotting times in the presence of therapeutic heparin. These latter two reagents have potential use in monitoring heparin therapy.